hair papilla cell growth medium  (PromoCell)

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Screening process to determine peptides with hair-restoring properties. ( A ) Human Follicle Dermal Papilla Cell (HFDPCs) viability of over 200 cell-penetrating peptides, with EGF as the positive control. The red dots mark the peptides with the highest cell viability. ( B ) Western Blot results showing the upregulation of β-catenin and p-ERK proteins of 22 chosen peptides compared to the control. Peptide No.218 (in red rectangle) showed the highest expression level of β-catenin and p-ERK proteins, and was chosen for subsequent analyses. ( C ) Cell-penetrating capability of DualPep-ALO was evaluated using FITC-conjugated peptides in HFDPCs. Cells were treated with FITC-DualPep-ALO or FITC-TAT (2.5 µM, 2 h), followed by extensive washing and trypsinization to remove membrane-bound peptides. Intracellular fluorescence intensity was quantified by flow cytometry. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Positive Control, Western Blot, Control, Expressing, Membrane, Fluorescence, Flow Cytometry

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Effects of DualPep-ALO on cell viability. ( A ) Mouse macrophage viability after 24-hour treatment with various concentrations of DualPep-ALO (0.64, 3.2, 16, 80 µM), compared with L-NMMA (25 µM). ( B ) Human Follicle Dermal Papilla Cells viability after treatment with DualPep-ALO (3.2, 16, 80 µM) for 24, 48, and 72 hours, compared with minoxidil (20 µM). Data are presented as mean ± SD. *p < 0.05 vs. negative control at the same time point. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Negative Control

Journal: BMC Biotechnology

Article Title: A novel cell-penetrating peptide supports hair follicle growth through anti-inflammatory and growth factor–associated mechanisms in preclinical models

doi: 10.1186/s12896-026-01130-4

Figure Lengend Snippet: Antioxidant enzyme activities in Human Follicle Dermal Papilla Cells (HFPDCs) treated with DualPep-ALO. DualPep-ALO (3.2, 16, 80 µM) enhanced the activities of ( A ) Superoxide dismutase (SOD) and ( B ) catalase (CAT) in HFPDCs after oxidative stress induction with H 2 O 2 (400 µM). L-Ascorbic acid (100 µM) was used as a positive control. Data are expressed as mean ± SD. *p < 0.05 vs. negative control; #p < 0.05 vs. H 2 O 2 -treated group. Each experiment was performed three times

Article Snippet: HFDPCs were cultured in Human Follicle Dermal Papilla Cell Growth Medium (PromoCell, Heidelberg, Germany) supplemented with fetal calf serum, bovine pituitary extract, basic fibroblast growth factor, and insulin.

Techniques: Positive Control, Negative Control